Redox regulation of soluble guanylyl cyclase
نویسندگان
چکیده
منابع مشابه
Regulation of soluble guanylyl cyclase by phosphorylation
Results In vitro kinase assays revealed that the α1, but not the β1, subunit of sGC is a PKG substrate and that the phosphorylation site is located within the first 360 residues of the α1. A constitutively active form of PKG stimulated incorporation of 32P into the α1 subunit in vivo. In addition, PKG could be detected in sGC immunoprecipitates, suggesting that the two proteins interact in cell...
متن کاملThiol-dependent redox modulation of soluble guanylyl cyclase
Background Following prolonged exposure to NO, soluble guanylyl cyclase (sGC) becomes desensitized and fails to respond to additional NO stimulation. We showed that sGC is desensitized by S-nitrosylation in vitro, in primary smooth muscle cells (SMC) and in tissues and identified two cysteines (Cys) targeted by this post-translational modification that are involved in sGC desensitization [1]. W...
متن کاملRegulation of soluble guanylyl cyclase: looking beyond NO.
NEUROTRANSMISSION, PLATELET FUNCTION, skeletal muscle and vascular and nonvascular smooth muscle, and numerous other cellular functions are significantly regulated by the principal enzyme initially transducing nitric oxide (NO) signals, soluble guanylyl cyclase (sGC) (8). The better-characterized active forms of this enzyme exist as a heterodimer, comprising an and a heme-containing -sGC subuni...
متن کاملHuman recombinant soluble guanylyl cyclase: expression, purification, and regulation.
The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...
متن کاملNucleotidyl cyclase activity of soluble guanylyl cyclase in intact cells.
Soluble guanylyl cyclase (sGC) is activated by nitric oxide (NO) and generates the second messenger cyclic GMP (cGMP). Recently, purified sGC α1β1 has been shown to additionally generate the cyclic pyrimidine nucleotides cCMP and cUMP. However, since cyclic pyrimidine nucleotide formation occurred only the presence of Mn(2+) but not Mg(2+), the physiological relevance of these in vitro findings...
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ژورنال
عنوان ژورنال: Nitric Oxide
سال: 2018
ISSN: 1089-8603
DOI: 10.1016/j.niox.2018.03.013